rnascope probe-egfp Search Results


sr 95531 hydrobromide  (Bio-Techne corporation)
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Sr 95531 Hydrobromide, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnascope probe egfp-c3  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope probe egfp-c3
KEY RESOURCES TABLE
Rnascope Probe Egfp C3, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnascope probe- mm-slc32a1-c2  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope probe- mm-slc32a1-c2
KEY RESOURCES TABLE
Rnascope Probe Mm Slc32a1 C2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aav9 camkii gfp addgene rrid addgene 105541 commercial assay  (Addgene inc)
93
Addgene inc aav9 camkii gfp addgene rrid addgene 105541 commercial assay
Figure 6. Genetic knockdown of <t>Htr1a</t> receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of <t>AAV9-CamKII-GCaMP6s</t> and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing
Aav9 Camkii Gfp Addgene Rrid Addgene 105541 Commercial Assay, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aav9 camkii gfp addgene rrid addgene 105541 commercial assay/product/Addgene inc
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rnascope probe egfp  (Thermo Fisher)
86
Thermo Fisher rnascope probe egfp
( A ) <t>RNAscope</t> multiplex fluorescent hybridization for tdTOM , GFP and Car4 transcripts on P30 brains showing that L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ INs express Car4 at significantly higher levels (orange outline) as compared to Htr3a -GFP+ INs negative for tdTOM (green outline) (***p<0.0001; Mann-Whitney test). ( B ) Example of a L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ cell (arrowhead) or Htr3a -GFP+ INs negative for tdTOM (open arrowheads) checked before patching (left image). Example of a patched Hmx3 ; tdTOM+/ Htr3a -GFP+ IN (middle and right images, arrowhead). ( C ) Illustrative reconstruction of a Hmx3 ; tdTOM+/ Htr3a -GFP+ IN in L1 displaying the characteristic morphology of an elongated NGC with dense axonal ramifications restricted to L1. ( D ) Illustrative reconstruction of a Htr3a -GFP+ IN negative for tdTOM in L1 displaying the characteristic morphology of single bouquet-like cell (SBC) with axonal ramifications extending deep into L5. ( E ) Illustrative traces from recorded Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green), showing the first action potentials (APs) at rheobase and trains of APs at higher current injections. ( F ) Superimposed single AP traces at rheobase of all Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green). Thick traces correspond to type 1A and type 2A examples in E. ( G ) Same traces as in ( F ), aligned to the AP, with a lower time scale. Thin lines are individual cell traces and thick lines are trace averages. The average traces on the right are aligned to the threshold potential (Vthr). ( H ) Plots of AP peak amplitude (Peak; ***p<0.0001; unpaired t-test), after hyperpolarization potential amplitude (AHP; ***p<0.0001; unpaired t-test) and membrane resistance (Rm; *p=0.0318; Mann-Whitney test) showing significant differences between the two cell types. ( I ) Absolute linear weights assigned by the classification model trained on all cells with standardized electrophysiological properties. ( J ) Prediction probabilities estimated by the classifier on the cell left out in the leave-one-out-cross-validation (LOOCV) loop. Cells are ordered on the x-axis by origin and prediction value, and the color code reflect their origin. Cells above the probability threshold 0.5 are more likely to be Hmx3 -derived according to the model. Scale bars: 10 µm A; 20 µm in B; 100 µm C, D. 10.7554/eLife.32017.025 Figure 6—source data 1. Detailed counts of cells expressing Car4 quantified in in the different experimental conditions. 10.7554/eLife.32017.026 Figure 6—source data 2. Electrophysiological properties of cells quantified in .
Rnascope Probe Egfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gabrb3 acd  (Addgene inc)
91
Addgene inc gabrb3 acd
( A ) <t>RNAscope</t> multiplex fluorescent hybridization for tdTOM , GFP and Car4 transcripts on P30 brains showing that L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ INs express Car4 at significantly higher levels (orange outline) as compared to Htr3a -GFP+ INs negative for tdTOM (green outline) (***p<0.0001; Mann-Whitney test). ( B ) Example of a L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ cell (arrowhead) or Htr3a -GFP+ INs negative for tdTOM (open arrowheads) checked before patching (left image). Example of a patched Hmx3 ; tdTOM+/ Htr3a -GFP+ IN (middle and right images, arrowhead). ( C ) Illustrative reconstruction of a Hmx3 ; tdTOM+/ Htr3a -GFP+ IN in L1 displaying the characteristic morphology of an elongated NGC with dense axonal ramifications restricted to L1. ( D ) Illustrative reconstruction of a Htr3a -GFP+ IN negative for tdTOM in L1 displaying the characteristic morphology of single bouquet-like cell (SBC) with axonal ramifications extending deep into L5. ( E ) Illustrative traces from recorded Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green), showing the first action potentials (APs) at rheobase and trains of APs at higher current injections. ( F ) Superimposed single AP traces at rheobase of all Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green). Thick traces correspond to type 1A and type 2A examples in E. ( G ) Same traces as in ( F ), aligned to the AP, with a lower time scale. Thin lines are individual cell traces and thick lines are trace averages. The average traces on the right are aligned to the threshold potential (Vthr). ( H ) Plots of AP peak amplitude (Peak; ***p<0.0001; unpaired t-test), after hyperpolarization potential amplitude (AHP; ***p<0.0001; unpaired t-test) and membrane resistance (Rm; *p=0.0318; Mann-Whitney test) showing significant differences between the two cell types. ( I ) Absolute linear weights assigned by the classification model trained on all cells with standardized electrophysiological properties. ( J ) Prediction probabilities estimated by the classifier on the cell left out in the leave-one-out-cross-validation (LOOCV) loop. Cells are ordered on the x-axis by origin and prediction value, and the color code reflect their origin. Cells above the probability threshold 0.5 are more likely to be Hmx3 -derived according to the model. Scale bars: 10 µm A; 20 µm in B; 100 µm C, D. 10.7554/eLife.32017.025 Figure 6—source data 1. Detailed counts of cells expressing Car4 quantified in in the different experimental conditions. 10.7554/eLife.32017.026 Figure 6—source data 2. Electrophysiological properties of cells quantified in .
Gabrb3 Acd, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnascope protease plus  (Advanced Cell Diagnostics Inc)
90
Advanced Cell Diagnostics Inc rnascope protease plus
( A ) <t>RNAscope</t> multiplex fluorescent hybridization for tdTOM , GFP and Car4 transcripts on P30 brains showing that L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ INs express Car4 at significantly higher levels (orange outline) as compared to Htr3a -GFP+ INs negative for tdTOM (green outline) (***p<0.0001; Mann-Whitney test). ( B ) Example of a L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ cell (arrowhead) or Htr3a -GFP+ INs negative for tdTOM (open arrowheads) checked before patching (left image). Example of a patched Hmx3 ; tdTOM+/ Htr3a -GFP+ IN (middle and right images, arrowhead). ( C ) Illustrative reconstruction of a Hmx3 ; tdTOM+/ Htr3a -GFP+ IN in L1 displaying the characteristic morphology of an elongated NGC with dense axonal ramifications restricted to L1. ( D ) Illustrative reconstruction of a Htr3a -GFP+ IN negative for tdTOM in L1 displaying the characteristic morphology of single bouquet-like cell (SBC) with axonal ramifications extending deep into L5. ( E ) Illustrative traces from recorded Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green), showing the first action potentials (APs) at rheobase and trains of APs at higher current injections. ( F ) Superimposed single AP traces at rheobase of all Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green). Thick traces correspond to type 1A and type 2A examples in E. ( G ) Same traces as in ( F ), aligned to the AP, with a lower time scale. Thin lines are individual cell traces and thick lines are trace averages. The average traces on the right are aligned to the threshold potential (Vthr). ( H ) Plots of AP peak amplitude (Peak; ***p<0.0001; unpaired t-test), after hyperpolarization potential amplitude (AHP; ***p<0.0001; unpaired t-test) and membrane resistance (Rm; *p=0.0318; Mann-Whitney test) showing significant differences between the two cell types. ( I ) Absolute linear weights assigned by the classification model trained on all cells with standardized electrophysiological properties. ( J ) Prediction probabilities estimated by the classifier on the cell left out in the leave-one-out-cross-validation (LOOCV) loop. Cells are ordered on the x-axis by origin and prediction value, and the color code reflect their origin. Cells above the probability threshold 0.5 are more likely to be Hmx3 -derived according to the model. Scale bars: 10 µm A; 20 µm in B; 100 µm C, D. 10.7554/eLife.32017.025 Figure 6—source data 1. Detailed counts of cells expressing Car4 quantified in in the different experimental conditions. 10.7554/eLife.32017.026 Figure 6—source data 2. Electrophysiological properties of cells quantified in .
Rnascope Protease Plus, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnascope 2.5 ls negative control probe_dapb  (Advanced Cell Diagnostics Inc)
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rnascope 2.5 ls probe - rnmbp-o1-c2  (Advanced Cell Diagnostics Inc)
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Systematic analysis of purified astrocytes after SCI unveils Zeb2os function during astrogliosis

doi: 10.1016/j.celrep.2021.108721

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: RNAscope Probe EGFP-C3 , Advanced Cell Diagnostics , 400281-C3.

Techniques: Virus, shRNA, Plasmid Preparation, Purification, Recombinant, Multiplex Assay, Transfection, RNAscope, Negative Control, Sequencing, Software, Flow Cytometry, Fluorescence, Microscopy, Laser-Scanning Microscopy

Figure 6. Genetic knockdown of Htr1a receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing

Journal: eLife

Article Title: Serotonin modulates infraslow oscillation in the dentate gyrus during Non-REM sleep

doi: 10.7554/elife.100196

Figure Lengend Snippet: Figure 6. Genetic knockdown of Htr1a receptors in dentate gyrus (DG) impairs infraslow oscillatory (ISO) and memory performance. (A) Schematic representation of the experimental design. A mix of AAV9-CamKII-GCaMP6s and AAV1-hSyn-Cre was injected into the DG of Htr1aflox/flox mice. (B) Representative example showing photometry and EEG recordings in the DG of a control mouse injected with AAV9-CaMKII-GCaMP6s alone. Right, Fourier transformation of calcium activity during wake (blue) non-rapid eye movement (NREM) sleep (red). (C) A representative example showing

Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain (C57BL/6 J) C57BL/6 J mouse JAX: #000664 RRID:IMSR_JAX:000664 Strain (Dock10Cre) Dock10Cre mouse MGI:6117432 N/A transgene insertion, by Susumu Tonegawa Strain (Drd2Cre) Drd2Cre mouse GENSAT MMRRC:032108- UCD Strain (Slc6a4Cre) Slc6a4Cre mouse JAX: #014554 RRID:IMSR_JAX:014554 Common name: SERT- Cre Strain (Htr1aflox/flox) Htr1aflox/flox mouse Rene Hen at Columbia University N/A Generated by Rene Hen Strain (pAAV.Syn.Flex.GCaMP6s.WPRE.SV40) AAV1- SYN- FLEX- GCaMP6s Addgene RRID:Addgene_100845 Strain (AAV.CamKII.GCaMP6s.WPRE.SV40) AAV9- CamKIIa- GCaMP6s Addgene RRID:Addgene_107790 Strain (AAVdj- syn- jGCaMP7b) AAVdj- syn- jGCaMP7b Gene Vector and Virus Core at Stanford University RRID:Addgene_104489 Custom- made at Stanford virus core Strain (AAV9- hSyn- GRAB5- HT2h) AAV9- hSyn- GRAB5- HT2h Vigene Biosciences Cat#YL10097- AV9 Strain (pENN.AAV.hSyn.Cre.WPRE.hGH) AAV1- hSyn- Cre Addgene RRID:Addgene_105553 Strain (pENN.AAV.CamKII.HI.GFP- Cre.WPRE.SV40) AAV9- CaMKII- Cre- GFP Addgene RRID:Addgene_105551 Strain (pENN.AAV.CamKII0.4.eGFP.WPRE.rBG) AAV9- CaMKII- GFP Addgene RRID:Addgene_105541 Commercial assay (Htr1a RNAscope probe) Htr1a mouse RNAscope probe Advanced Cell Diagnostics Cat#312301 Commercial assay (EGFP RNAscope probe) EGFP RNAscope probe Advanced Cell Diagnostics Cat#400281

Techniques: Knockdown, Injection, Control, Transformation Assay, Activity Assay

( A ) RNAscope multiplex fluorescent hybridization for tdTOM , GFP and Car4 transcripts on P30 brains showing that L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ INs express Car4 at significantly higher levels (orange outline) as compared to Htr3a -GFP+ INs negative for tdTOM (green outline) (***p<0.0001; Mann-Whitney test). ( B ) Example of a L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ cell (arrowhead) or Htr3a -GFP+ INs negative for tdTOM (open arrowheads) checked before patching (left image). Example of a patched Hmx3 ; tdTOM+/ Htr3a -GFP+ IN (middle and right images, arrowhead). ( C ) Illustrative reconstruction of a Hmx3 ; tdTOM+/ Htr3a -GFP+ IN in L1 displaying the characteristic morphology of an elongated NGC with dense axonal ramifications restricted to L1. ( D ) Illustrative reconstruction of a Htr3a -GFP+ IN negative for tdTOM in L1 displaying the characteristic morphology of single bouquet-like cell (SBC) with axonal ramifications extending deep into L5. ( E ) Illustrative traces from recorded Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green), showing the first action potentials (APs) at rheobase and trains of APs at higher current injections. ( F ) Superimposed single AP traces at rheobase of all Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green). Thick traces correspond to type 1A and type 2A examples in E. ( G ) Same traces as in ( F ), aligned to the AP, with a lower time scale. Thin lines are individual cell traces and thick lines are trace averages. The average traces on the right are aligned to the threshold potential (Vthr). ( H ) Plots of AP peak amplitude (Peak; ***p<0.0001; unpaired t-test), after hyperpolarization potential amplitude (AHP; ***p<0.0001; unpaired t-test) and membrane resistance (Rm; *p=0.0318; Mann-Whitney test) showing significant differences between the two cell types. ( I ) Absolute linear weights assigned by the classification model trained on all cells with standardized electrophysiological properties. ( J ) Prediction probabilities estimated by the classifier on the cell left out in the leave-one-out-cross-validation (LOOCV) loop. Cells are ordered on the x-axis by origin and prediction value, and the color code reflect their origin. Cells above the probability threshold 0.5 are more likely to be Hmx3 -derived according to the model. Scale bars: 10 µm A; 20 µm in B; 100 µm C, D. 10.7554/eLife.32017.025 Figure 6—source data 1. Detailed counts of cells expressing Car4 quantified in in the different experimental conditions. 10.7554/eLife.32017.026 Figure 6—source data 2. Electrophysiological properties of cells quantified in .

Journal: eLife

Article Title: Neurogliaform cortical interneurons derive from cells in the preoptic area

doi: 10.7554/eLife.32017

Figure Lengend Snippet: ( A ) RNAscope multiplex fluorescent hybridization for tdTOM , GFP and Car4 transcripts on P30 brains showing that L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ INs express Car4 at significantly higher levels (orange outline) as compared to Htr3a -GFP+ INs negative for tdTOM (green outline) (***p<0.0001; Mann-Whitney test). ( B ) Example of a L1 Hmx3 ; tdTOM+/ Htr3a -GFP+ cell (arrowhead) or Htr3a -GFP+ INs negative for tdTOM (open arrowheads) checked before patching (left image). Example of a patched Hmx3 ; tdTOM+/ Htr3a -GFP+ IN (middle and right images, arrowhead). ( C ) Illustrative reconstruction of a Hmx3 ; tdTOM+/ Htr3a -GFP+ IN in L1 displaying the characteristic morphology of an elongated NGC with dense axonal ramifications restricted to L1. ( D ) Illustrative reconstruction of a Htr3a -GFP+ IN negative for tdTOM in L1 displaying the characteristic morphology of single bouquet-like cell (SBC) with axonal ramifications extending deep into L5. ( E ) Illustrative traces from recorded Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green), showing the first action potentials (APs) at rheobase and trains of APs at higher current injections. ( F ) Superimposed single AP traces at rheobase of all Hmx3 ; tdTOM+/ Htr3a -GFP+ INs (orange) and Htr3a -GFP+ INs negative for tdTOM (green). Thick traces correspond to type 1A and type 2A examples in E. ( G ) Same traces as in ( F ), aligned to the AP, with a lower time scale. Thin lines are individual cell traces and thick lines are trace averages. The average traces on the right are aligned to the threshold potential (Vthr). ( H ) Plots of AP peak amplitude (Peak; ***p<0.0001; unpaired t-test), after hyperpolarization potential amplitude (AHP; ***p<0.0001; unpaired t-test) and membrane resistance (Rm; *p=0.0318; Mann-Whitney test) showing significant differences between the two cell types. ( I ) Absolute linear weights assigned by the classification model trained on all cells with standardized electrophysiological properties. ( J ) Prediction probabilities estimated by the classifier on the cell left out in the leave-one-out-cross-validation (LOOCV) loop. Cells are ordered on the x-axis by origin and prediction value, and the color code reflect their origin. Cells above the probability threshold 0.5 are more likely to be Hmx3 -derived according to the model. Scale bars: 10 µm A; 20 µm in B; 100 µm C, D. 10.7554/eLife.32017.025 Figure 6—source data 1. Detailed counts of cells expressing Car4 quantified in in the different experimental conditions. 10.7554/eLife.32017.026 Figure 6—source data 2. Electrophysiological properties of cells quantified in .

Article Snippet: Recombinant DNA reagent , RNAScope Probe-EGFP , Affimetrix , 400281 , .

Techniques: Multiplex Assay, Hybridization, MANN-WHITNEY, Derivative Assay, Expressing

Journal: eLife

Article Title: Neurogliaform cortical interneurons derive from cells in the preoptic area

doi: 10.7554/eLife.32017

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , RNAScope Probe-EGFP , Affimetrix , 400281 , .

Techniques: Recombinant, Plasmid Preparation, Concentration Assay, Sequencing, Fluorescence, Multiplex Assay, Software

Journal: eLife

Article Title: Neurogliaform cortical interneurons derive from cells in the preoptic area

doi: 10.7554/eLife.32017

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , RNAScope Probe-EGFP , Affimetrix , 400281 , .

Techniques: Recombinant, Plasmid Preparation, Concentration Assay, Sequencing, Fluorescence, Multiplex Assay, Software